 .      
  


       ,        .            .                        .             ,            .

       ,         ,          ,     .





 .      





 


-      

  

  - 

  

-      

  

    

   

  

     

   

  

  - 

   

   

   

    

5-TAMRA  5-carboxytetramethylrhodamine

CCD- (-)  charge-coupled device (   )

DABCO  

DAPI  4,6--2-

DMD  digital micromirror device (  )

EB   

FITC  

FLAP  Fluorescence Localization After Photobleaching (   )

FLIM  fluorescence lifetime imaging microscopy (     )

FLIP  Fluorescence Lossin Photobleaching (    )

FRAP  Fluorescence Recovery After Photobleachin (   )

FRET  Frster (Fluorescence) Resonance Energy Transfer (Ը ()   )

GPDH  

LDH  

PBFI  potassium-binding benzofuran isophtalate

PPI   

PPDA  

RITC  

SBFI  sodium-binding benzofuran isophtalate

SDS   

SHIM  Second-harmonic imaging microscopy (     )

SNAP25  Sy Naptosomal-Associated Protein, 25 kD

SNARE  Soluble N-ethylmaleimide-sensitive factor Attachment protein Receptor

TRITC  -5(6)  







      ,    ,             .   ,                 .  ,  ,   , ,   ,        , , ,             .  ,        ,      ,                  ,         ,     .      ,   ,   .              ,     .    ,   ,   ,      ,          .  -             ,    ,        ,    ,    ,       .         ,          .  -            ,        ,       ,    .

         ,   ,      ,   Zeiss (),      LSM 710  LSM 510 Meta.                  .

                     ,         .

 ,       ,        ( 14-15-00014).



. . 




 1.

          



       ,    ,          .       :      ,    ,      .          ,         .   .




1.1.  


 ( )[1 -          ,         ,   컠          .10]   ,             .                   . ,        ;      .            .                ( ):








 ?     ; v  ; ?   ; h   ;    .

          ,        .   ,     ,    , ,  ,               .             .  (2011).

       . ,        FITC (fluorescein-5-isothiocyanate)     l


 = 492 ,       l


 = 518 .   , 5-TAMRA (5-carboxytetramethylrhodamine),  l


 = 543   l


 = 570 .        ,    .

     ,      ,    ,               ,      . ,            ( ),       . ,         900         450  300 .

             .   (Q)        (F)     (I


)   (I)  :








,       -  .           .   ,  ,       .

             ,           -

   1.       ? 





?  





.           . ,          ,        .  ,     50% ,  .          .       ( )         ( . ., 2011).

        ,            ,     .     (?)  :








       ; k      .

      10 .

    ,      .      :     ,  ,  ,   .        ,      (  , ).          .     ,      ,            ,    .           .              .        .      ,    , ,  , ,  , , , , , .  ,         ,  (    )       - , ,    (     ).

    .        .          ,            .             .      r  :








 Iv  Ih               .

     ,             .            ,    .  ,          (          ).  ,           ,         .                .




1.2.   


        .  ,            .      ,      ( 1907  1938.   ),      (1903)  .     (1911) Oskar Heimstdt               .              .     Philipp Ellinger  August Hirt  1929.,       .                 ,         .          (60- . XX.),             .  ,     ,   .

       .1.

       .        ,     .       ().            ,  .   (    (  )),       , ,     ,    ,              .

  .        :    (,    ),   .       ,                   .     ,              ,    .         50  200 W.            ,     ,           .






. 1.    



               ,    .               ,    ,     .          ,       .        ,      (   ).

         (metal halide lamp).     .        ,       ( , ,   .),   (   )   .          ,   ,   ,     .          .    ,          ,          (, ).  ,    ,   ,       .

             ,    ,      ,   .      ,    .

                        .            .        ,     .    ,      ,           .

.

     ,          ,     . ,       , ,        (,   BP 560/40           BP 370/40, BP 474/28, BP 585/35    Carl Zeiss).

  ( )    ,   ,      ,       .      ,     .           ( 10  200)         . , PbCl2, TiO2, ZnS ( 2,2  2,3)  MgF


, SiO


, Na


AlF


 ( 1,3  1,4).      (   ),        .              .

  (band pass BP).         (shot pass filter,  SP filter)    (long pass filter,  LP filter) .    ,   ,  LP-, ,   ,  .   ,   ,          .         . ,     BP 560/40     BP 630/75 .

           .                ,      .             ,          .      ,  ,       .        .




  .


   .

   ,     (https://www.litres.ru/raznoe/molekulyarnaya-morfologiya-metody-fluorescentnoy-i-konfokalnoy-laze/)  .

      Visa, MasterCard, Maestro,    ,   ,     ,  PayPal, WebMoney, ., QIWI ,       .



notes



1


         ,         ,   컠          .10


